rat fabp4 elisa kit  (Boster Bio)

Journal: Cell Death & Disease

Article Title: RETRACTED ARTICLE: FABP4 contributes to renal interstitial fibrosis via mediating inflammation and lipid metabolism

doi: 10.1038/s41419-019-1610-5

Figure Lengend Snippet: a , b The protein levels of FABP4 in NRK-52E and NRK-49F cells based on immunofluorescence staining and western blotting assay ( n = 3). c , d The protein levels of FABP4 in mice and rats based on immunofluorescence staining and western blotting assay ( n = 3). e Serum protein levels of FABP4 in mice and rats based on Elisa assay ( n = 7). Data are presented as the mean ± SD. ** p < 0.01 compared with sham groups or control groups

Article Snippet: Mouse and Rat FABP4 ELISA Kits were purchased from Boster Biological Technology Co., Ltd. (California, USA).

Techniques: Immunofluorescence, Staining, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Cell Death & Disease

Article Title: RETRACTED ARTICLE: FABP4 contributes to renal interstitial fibrosis via mediating inflammation and lipid metabolism

doi: 10.1038/s41419-019-1610-5

Figure Lengend Snippet: a The protein levels of FABP4 and PPARγ based on immunefluorescence staining (400× magnification) and western blotting assay in FABP4 siRNA transfected NRK-52E and NRK-49F cells. b The mRNA levels of IL-1β, IL-6, and TNF-α, and the protein levels of p65 and ICAM-1 in NRK-52E and NRK-49F cells after FABP4 siRNA transfection. c The protein levels of EHHADH, ACOX1, SCP-2, CPT1, ACADM, and ACADL in NRK-52E and NRK-49F cells after FABP4 siRNA transfection. d The mRNA levels of α-SMA, COL1A1 and COL3A1 in NRK-52E and NRK-49F cells after FABP4 siRNA transfection and the protein levels of α-SMA and COL1A based on immunofluorescence assay (400× magnification) in NRK-52E and NRK-49F cells after FABP4 siRNA transfection. e The levels of TC, TG, and FFAs in NRK-52E and NRK-49F cells after FABP4 siRNA transfection and the lipid droplets based on Oil O Red staining (400× magnification) in NRK-52E and NRK-49F cells after FABP4 siRNA transfection. Data are presented as the mean ± SD ( n = 3). ** p < 0.01 control groups compared with LPS-groups; ## p < 0.01 and # p < 0.05 LPS groups compared with FABP4 siRNA groups

Article Snippet: Mouse and Rat FABP4 ELISA Kits were purchased from Boster Biological Technology Co., Ltd. (California, USA).

Techniques: Staining, Western Blot, Transfection, Immunofluorescence

Journal: Cell Death & Disease

Article Title: RETRACTED ARTICLE: FABP4 contributes to renal interstitial fibrosis via mediating inflammation and lipid metabolism

doi: 10.1038/s41419-019-1610-5

Figure Lengend Snippet: a The protein levels of FABP4, PPARγ, p65, and ICAM based on immunefluorescence staining (400× magnification) and western blotting assay after treatment by FABP4 inhibitor in NRK-52E and NRK-49F cells. b The protein levels of EHHADH, ACOX1, SCP-2, CPT1, ACADM, and ACADL in NRK-52E and NRK-49F cells treated by FABP4 inhibitor. c The protein levels of α-SMA and COL1A based on immunofluorescence assay (400× magnification), and the mRNA levels of α-SMA, COL1A1, and COL3A1 in NRK-52E and NRK-49F cells after treatment by FABP4 inhibitor in NRK-52E and NRK-49F cells. d The lipid droplets based on Oil O Red staining (400× magnification) and the levels of TC, TG, and FFAs in NRK-52E and NRK-49F cells after FABP4 inhibitor treatment. Data are presented as the mean ± SD ( n = 3). ** p < 0.01 control groups compared with LPS groups, ## p < 0.01 and # p < 0.05 LPS groups compared with FABP4 inhibitor groups

Article Snippet: Mouse and Rat FABP4 ELISA Kits were purchased from Boster Biological Technology Co., Ltd. (California, USA).

Techniques: Staining, Western Blot, Immunofluorescence

Journal: Cell Death & Disease

Article Title: RETRACTED ARTICLE: FABP4 contributes to renal interstitial fibrosis via mediating inflammation and lipid metabolism

doi: 10.1038/s41419-019-1610-5

Figure Lengend Snippet: a The protein levels of FABP4 based on immunofluorescence staining (400× magnification) and western blotting assay in FABP4 KO mice. b The protein levels of PPARγ based on immunofluorescence staining (400× magnification) and western blotting assay in FABP4 KO mice. c The mRNA levels of IL-1β, IL-6, and TNF-α in FABP4 KO mice. d The protein levels of p65, ICAM-1, EHHADH, ACOX1, SCP-2, CPT1, ACADM, and ACADL in FABP4 KO mice. Data are presented as the mean ± SD ( n = 3). ** p < 0.01 Sham group compared with UUO mice; ## p < 0.01 and # p < 0.05 UUO WT mice compared with UUO FABP4 −/− mice

Article Snippet: Mouse and Rat FABP4 ELISA Kits were purchased from Boster Biological Technology Co., Ltd. (California, USA).

Techniques: Immunofluorescence, Staining, Western Blot

Journal: Cell Death & Disease

Article Title: RETRACTED ARTICLE: FABP4 contributes to renal interstitial fibrosis via mediating inflammation and lipid metabolism

doi: 10.1038/s41419-019-1610-5

Figure Lengend Snippet: a The serum levels of Cr, BUN, and H&E staining (400× original magnification) of the kidney tissue in FABP4 KO mice ( n = 5). b Degree of fibrosis based on Masson and Sirius Red staining (400× original magnification), and Sirius Red polarized light observation (400× original magnification) in FABP4 KO mice. c The mRNA levels of α-SMA, COL1A1, COL3A1, and the protein levels of α-SMA and COL1A based on immunofluorescence staining (400× magnification) in FABP4 KO mice ( n = 3). d The lipid droplets based on Oil O Red staining (400× original magnification), and the levels of TG, TC, and FFA in FABP4 KO mice. Data are presented as the mean ± SD. ** p < 0.01 and * p < 0.05 Sham group compared with UUO FABP4 KO mice; ## p < 0.01 and # p < 0.05 UUO WT mice group compared with UUO FABP4 −/− mice

Article Snippet: Mouse and Rat FABP4 ELISA Kits were purchased from Boster Biological Technology Co., Ltd. (California, USA).

Techniques: Staining, Immunofluorescence

Journal: Cell Death & Disease

Article Title: RETRACTED ARTICLE: FABP4 contributes to renal interstitial fibrosis via mediating inflammation and lipid metabolism

doi: 10.1038/s41419-019-1610-5

Figure Lengend Snippet: FABP4 downregulated PPAR-γ expression, up-regulated the protein levels of p65 and ICAM-1, and decreased the protein levels of ACADM, ACADL, SCP-2, CPT1, EHHADH, and ACOX1 to promote inflammation and lipid metabolism

Article Snippet: Mouse and Rat FABP4 ELISA Kits were purchased from Boster Biological Technology Co., Ltd. (California, USA).

Techniques: Expressing

Journal: Endocrinology

Article Title: In utero exposure to the endocrine disruptor di-(2-ethylhexyl) phthalate induces long-term changes in gene expression in the adult male adrenal gland.

doi: 10.1210/en.2013-1921

Figure Lengend Snippet: Figure 6. Serum markers that correlate with in utero exposure to DEHP. A, Heatmap of differentially expressed genes at PND21 and PND60 in offspring of rats exposed in utero to 100- or 300-mg DEHP/kgd was constructed to identify genes altered at both time points and doses. Selected gene targets (bold) were used to evaluate putative serum markers. Serum levels of AQP7 (B), FABP4 (C), and PCK1 (D) in adult rats exposed in utero to the indicated doses of DEHP. Data in C and D are means SD (n 4); **, ANOVA, P .01. E, Relationship between FABP4 and PCK1 adult rat serum levels to in utero DEHP exposure doses used. FABP4, correlation coefficient 0.37, P .49. PCK1, correlation coefficient 0.77, P .1.

Article Snippet: ELISAs were used to quantify aquaporin 7 (AQP7) (catalog number CSB-EL001967RA; Cusabio Biotech, Cedarlane Laboratories), fatty acid-binding protein 4 (FABP4) (catalog number CSB-E13027r; Cusabio Biotech), and phosphoenolpyruvate carboxykinase I (PCK1) (catalog number sE90936Ra; USCN Life Science, Inc, Cedarlane Laboratories), according to the instructions of each manufacturer.

Techniques: In Utero, Construct